Solubilization of V-ATPase transmembrane peptides by amphipol A8-35
نویسندگان
چکیده
منابع مشابه
Sequence-Specific Dimerization of a Transmembrane Helix in Amphipol A8-35
As traditional detergents might destabilize or even denature membrane proteins, amphiphilic polymers have moved into the focus of membrane-protein research in recent years. Thus far, Amphipols are the best studied amphiphilic copolymers, having a hydrophilic backbone with short hydrophobic chains. However, since stabilizing as well as destabilizing effects of the Amphipol belt on the structure ...
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Amphipols are short amphilic polymers designed for applications in membrane biochemistry and biophysics and used, in particular, to stabilize membrane proteins in aqueous solutions. Amphipol A8-35 was obtained by modification of a short-chain parent polymer (poly(acrylic acid); PAA) with octyl- and isopropylamine, to yield an amphiphilic product with an average molar mass of 9-10 kg x mol(-1) (...
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Amphipols are amphipathic polymers designed to replace or supplement detergents in membrane protein solution studies. Previous work has suggested both advantages and disadvantages to the use of a polyacrylate-based amphipol, A8-35, for studying the sarcoplasmic reticulum Ca2+-ATPase (SERCA1a). We investigated this issue further using a set of four amphipols with different chemical structures. P...
متن کاملSolubilization of Peptides
ity. The amino acid composition will provide a fi rst indication: before choosing a solvent, the sequence should be studied and the number of acidic, basic and neutral residues should be determined. The side-chain functionalities of acidic and basic amino acids will be charged at physiological pH. The nature of the Nand the C-terminal functionality has to be taken into consideration. The neutra...
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The structure and function of membrane proteins is partly determined by the interaction of these proteins with the lipids of the membrane. Peptides forming single membrane-spanning alpha-helices, such as the WALP peptide (acetyl-GWWL(AL)(n)WWA-amide), are good models for such interactions. This interaction can be studied by investigating the aggregation of peptides. If the peptides remain isola...
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ژورنال
عنوان ژورنال: Journal of Peptide Science
سال: 2008
ISSN: 1075-2617,1099-1387
DOI: 10.1002/psc.996